The Anti-phospho-Beclin-1 (Ser90) Antibody (CABP1254) is a valuable tool for researchers studying Beclin-1, a key regulator of autophagy, the cellular process responsible for degrading and recycling damaged organelles and proteins. This polyclonal antibody, raised against phosphorylated Ser90 of Beclin-1, is highly specific and sensitive, making it an ideal choice for Western blot and immunohistochemistry applications.Beclin-1 is known to be phosphorylated at Ser90 in response to various cellular stressors, such as nutrient deprivation and oxidative stress, leading to its activation and initiation of autophagy. The phosphorylation status of Beclin-1 at Ser90 is crucial for regulating autophagic activity and maintaining cellular homeostasis.
This antibody allows researchers to monitor the phosphorylation of Beclin-1 at Ser90 in different cell types and conditions, providing valuable insights into the role of autophagy in health and disease.By studying the phosphorylation of Beclin-1 at Ser90, researchers can gain a better understanding of the mechanisms underlying autophagy regulation and its implications for various pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders. This antibody is a powerful tool for investigating the role of Beclin-1 in autophagy and its potential as a therapeutic target for treating autophagy-related diseases.
Product Name:
Phospho-Beclin 1-Ser90 Rabbit Polyclonal Antibody
SKU:
CABP1254
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S90 of human Beclin 1 (NP_003757.1).
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8678
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-Beclin 1-Ser90 Rabbit pAb (CABP1254) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 60s.
