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Phospho-Beclin 1-Ser15 Rabbit Polyclonal Antibody (CABP1252)

SKU:
CABP1252
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ATG6
VPS30
beclin1
Beclin 1
BECN1
beclin-1
Research Area:
Immunology
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Beclin 1-Ser15 Rabbit Polyclonal Antibody
SKU:CABP1252
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1).
Sequence:QVSF V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:ATG6; VPS30; beclin1; Phospho-Beclin 1-Ser15
Positive Sample:NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane.
Calculated MW:52kDa
Observed MW:60kDa

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:8678
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (CABP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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