The Anti-phospho-ATR (T1989) Antibody (CABP1248) is a valuable tool for researchers studying the ATR protein, a key regulator of the DNA damage response. This antibody, produced in rabbits, is highly specific in detecting phosphorylation of ATR at threonine 1989 in human samples, making it an essential reagent for Western blot analysis.ATR is a crucial protein involved in sensing DNA damage and orchestrating the cellular responses necessary for DNA repair and cell cycle checkpoint activation. Phosphorylation of ATR at T1989 is a critical event in the activation of this protein, making the Anti-phospho-ATR (T1989) Antibody a valuable tool for studying the DNA damage response and its implications in cancer and other diseases.
By utilizing this antibody, researchers can gain insights into the role of ATR phosphorylation in DNA damage signaling pathways, cell cycle regulation, and genomic stability. This antibody is ideal for scientists conducting research in oncology, molecular biology, and DNA repair mechanisms. With its high specificity and sensitivity, the Anti-phospho-ATR (T1989) Antibody is a reliable resource for investigating the intricate mechanisms underlying cellular responses to DNA damage.
Product Name:
Phospho-ATR-T1989 Rabbit Polyclonal Antibody
SKU:
CABP1248
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T1989 of human ATR (NP_001175.2).
Sequence:
NETP P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
FRP1; MEC1; SCKL; FCTCS; SCKL1; Phospho-ATR-T1989
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, PML body.
Calculated MW:
301kDa
Observed MW:
300kDa
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Purification Method:
Affinity purification
Gene ID:
545
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (CABP1248) at 1:1000 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
