The Anti-Phospho-AMPKbeta1 (S182) Antibody (CABP1195) is a high-quality antibody designed for research involving AMPKbeta1, a key protein in the AMP-activated protein kinase (AMPK) signaling pathway. The antibody, raised in rabbits, is highly specific and sensitive in detecting phosphorylation of AMPKbeta1 at serine 182 in human samples, making it a valuable tool for studying cellular responses to energy stress and metabolic regulation.AMPK, a central regulator of energy balance, is activated by phosphorylation at multiple sites, including serine 182 on the beta1 subunit. Phosphorylation of AMPKbeta1 at S182 is known to play a critical role in the activation of AMPK and its downstream signaling pathways involved in glucose and lipid metabolism, making it a key target for research in metabolic disorders, diabetes, and cancer.
The Anti-Phospho-AMPKbeta1 (S182) Antibody (CABP1195) allows for the specific detection and analysis of phosphorylated AMPKbeta1 in various cell types and tissues, providing researchers with a reliable tool for studying the regulation of energy metabolism and cellular responses to stress. Its validated use in Western blot applications makes it an ideal choice for studies on the role of AMPK in metabolic diseases and potential therapeutic interventions targeting the AMPK signaling pathway.
Product Name:
Phospho-AMPKβ1-S182 Rabbit Polyclonal Antibody
SKU:
CABP1195
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S182 of human AMPKβ1 (NP_006244.2).
Sequence:
SSSP P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
AMPK; HAMPKb; Phospho-AMPKβ1-S182
Positive Sample:
C2C12
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, nucleoplasm, nucleus
Calculated MW:
30kDa
Observed MW:
38kDa
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Purification Method:
Affinity purification
Gene ID:
5564
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKβ1-S182 Rabbit pAb (CABP1195) at 1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
