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Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit Polyclonal Antibody (CABP1171)

SKU:
CABP1171
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AMPKa1/AMPKa2
Research Area:
Autophagy
$503
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Description

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Product Name:Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit Polyclonal Antibody
SKU:CABP1171
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5).
Sequence:LRTS C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AMPKa1/AMPKa2; Phospho-AMPKa1/AMPKa2-T183/T172
Positive Sample:293T,C2C12,C6
Cellular Localization:apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus
Calculated MW:64kDa/65kDa/62kDa
Observed MW:62kDa

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:5562/5563
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (CABP1171) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C6 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

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