The Anti-Phospho-AMPKa1/AMPKa2 (T183/T172) Antibody (CABP1171) is a valuable tool for researchers studying cellular signaling pathways involving AMP-activated protein kinase (AMPK). This antibody specifically targets the phosphorylated form of AMPKa1 and AMPKa2 at threonine residues 183 and 172, respectively. AMPK is a key regulator of cellular energy homeostasis and plays a crucial role in metabolic pathways. Phosphorylation of AMPK at these specific sites is known to modulate its activity, making this antibody essential for investigating the function and regulation of AMPK in various biological processes.The Anti-Phospho-AMPKa1/AMPKa2 (T183/T172) Antibody (CABP1171) is raised in rabbits and has been validated for use in Western blot applications.
Its high reactivity with human samples makes it a reliable tool for studying AMPK signaling in human cell lines and tissues. Researchers in the fields of metabolism, diabetes, cancer, and cardiovascular diseases will find this antibody invaluable for their studies.By enabling the specific detection and analysis of phosphorylated AMPKa1 and AMPKa2, this antibody opens up new avenues for understanding the role of AMPK in health and disease. Its use in research may ultimately lead to the development of novel therapeutic strategies targeting AMPK for the treatment of various metabolic and cardiovascular disorders.
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (CABP1171) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C6 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
