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Phospho-AKT1-T308 Rabbit Polyclonal Antibody (CABP1214)

SKU:
CABP1214
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AKT
CWS6
PKB
PKB-ALPHA
PRKBA
RAC
RAC-ALPHA
AKT1
€419
Frequently bought together:

Description

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Phospho-AKT1-T308 Rabbit Polyclonal Antibody (CABP1214)

The Anti-Phospho-Akt1 (T308) Antibody (CABP1214) is a highly specific and sensitive tool for researchers studying the Akt signaling pathway. Akt1 is a serine/threonine protein kinase that plays a crucial role in cell survival, proliferation, and metabolism. Phosphorylation of Akt1 at threonine 308 is a key regulatory step in its activation, making this antibody an important tool for studying the activation status of Akt1 in various cellular processes.This antibody is raised in rabbits and is highly reactive with human samples, making it an ideal choice for Western blot applications. Its specificity for Akt1 phosphorylated at threonine 308 ensures accurate and reliable detection of activated Akt1 in various cell types and experimental conditions.

Researchers can use this antibody to study the role of Akt1 activation in different physiological and pathological processes, including cancer, diabetes, and neurodegenerative diseases.By using the Anti-Phospho-Akt1 (T308) Antibody (CABP1214), researchers can gain valuable insights into the regulation and function of the Akt signaling pathway, ultimately leading to a better understanding of cellular processes and potentially identifying new targets for therapeutic intervention.

Product Name:Phospho-AKT1-T308 Rabbit Polyclonal Antibody
SKU:CABP1214
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T308 of human AKT (NP_005154.2).
Sequence:MKTF C
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-AKT1-T308
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:56kDa
Observed MW:60kDa

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using Phospho-AKT1-T308 antibody (CABP1214) at 1:1000 dilution.HeLa cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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