The Anti-Phospho-AKT1(S473)/AKT2(S474)/AKT3(S472) Antibody (CABP1208) is a valuable tool for researchers studying the phosphorylation of AKT proteins, key regulators of cell survival, proliferation, and metabolism. This antibody, produced in rabbits, is highly specific for detecting phosphorylation at specific sites on AKT1, AKT2, and AKT3 in human samples, making it ideal for use in Western blot applications.The phosphorylation of AKT proteins at these particular sites is known to play a crucial role in regulating various cellular processes, including apoptosis, cell growth, and glucose metabolism.
By targeting these specific phosphorylation sites, researchers can gain insights into the activation status of AKT signaling pathways in different cell types and disease conditions.The Anti-Phospho-AKT1(S473)/AKT2(S474)/AKT3(S472) Antibody (CABP1208) is a valuable tool for studying the intricate mechanisms by which AKT proteins regulate fundamental cellular processes. Its high specificity, sensitivity, and validated performance in Western blot applications make it an essential reagent for studies in cell biology, cancer research, and drug development aimed at targeting the AKT signaling pathway.
Product Name:
Phospho-Akt-S473 Rabbit Monoclonal Antibody
SKU:
CABP1208
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S473 of human AKT1 (NP_005154.2).
Sequence:
QFSY S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
AKT1/AKT2/AKT3; Phospho-Akt-S473
Positive Sample:
Jurkat+Calyculin A,NIH/3T3+Calyculin A,C6+Calyculin A
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Purification Method:
Affinity purification
Gene ID:
207/208/10000
Clone Number:
ARC5023-06
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (CABP1208) at 1:1000 dilution.Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
