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Phospho-Akt (Thr308) Rabbit Monoclonal Antibody (CABP1259)

SKU:
CABP1259
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AKT1/AKT2/AKT3
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Akt (Thr308) Rabbit Monoclonal Antibody
SKU:CABP1259
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T308 of human Akt (NP_005154.2).
Sequence:MKTF C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:AKT1/AKT2/AKT3; Phospho-Akt-T308
Positive Sample:NIH/3T3+Calyculin A,C6+Calyculin A
Conjugate:Unconjugated
Cellular Localization:Ciliary basal body, Cytoplasm, Cytosol, Microtubule cytoskeleton, Mitochondrion, Nucleoplasm, Nucleus, Plasma membrane, Spindle
Calculated MW:48kDa/55kDa/51kDa/54kDa
Observed MW:60kDa

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207/208/10000
Clone Number:ARC50457
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (CABP1259) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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