The PHF8 Polyclonal Antibody (CAB19780) is a valuable tool for researchers studying the PHF8 protein, which is known to play a role in epigenetic regulation and gene expression. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PHF8, a histone demethylase, is involved in the removal of methyl groups from histone proteins, impacting chromatin structure and gene transcription. Dysregulation of PHF8 has been linked to a variety of diseases, including certain cancers and neurodevelopmental disorders.
By targeting PHF8 with this antibody, researchers can gain insights into its function and potential therapeutic applications.The ability to detect and analyze PHF8 in various cell types makes this antibody ideal for studies in epigenetics, cancer biology, and developmental biology. By elucidating the role of PHF8 in these processes, researchers can further our understanding of disease mechanisms and potentially identify new targets for intervention.
Product Name:
PHF8 Rabbit Monoclonal Antibody
SKU:
CAB19780
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human PHF8 (Q9UPP1).
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
23133
Clone Number:
ARC2306
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using PHF8 antibody (CAB19780) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
