The Anti-PAX5 Antibody (CAB9607) is a highly specific and reliable tool for research involving the PAX5 protein, a transcription factor essential for B-cell development and function. Raised in rabbits, this polyclonal antibody exhibits excellent reactivity with human samples and is validated for use in various applications including Western blot and immunohistochemistry.PAX5, also known as B-cell specific activator protein (BSAP), plays a crucial role in B-cell differentiation and maturation, as well as in the regulation of other genes involved in B-cell development.
Dysregulation of PAX5 has been associated with various lymphoid malignancies, making it a valuable target for studies in cancer research and leukemia.By enabling the detection and analysis of PAX5 in various cell types and tissues, the Anti-PAX5 Antibody (CAB9607) provides researchers with a valuable tool for investigating the role of PAX5 in normal and pathological conditions. Its high specificity and sensitivity make it an essential reagent for studies aiming to understand the molecular mechanisms underlying B-cell development and lymphoid malignancies.
Product Name:
PAX5 Rabbit Monoclonal Antibody
SKU:
CAB9607
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PAX5 (Q02548).
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ALL3; BSAP; PAX-5; PAX5
Positive Sample:
Raji cells
Conjugate:
Unconjugated
Cellular Localization:
Nuclear
Calculated MW:
42kDa
Observed MW:
45kDa
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5079
Clone Number:
ARC1654
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using PAX5 Rabbit mAb (CAB9607) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
