The Parathyroid Hormone (PTH) Monoclonal Antibody (CAB9704) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various applications including Western blotting and immunohistochemistry.Parathyroid hormone plays a crucial role in bone metabolism, kidney function, and calcium absorption in the gut. Dysregulation of PTH levels can lead to bone disorders such as osteoporosis, as well as conditions like hyperparathyroidism and hypoparathyroidism.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Parathyroid Hormone (PTH) Monoclonal Antibody
SKU:
CAB9704
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1709
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FIH1, PTH1, Parathyroid Hormone (PTH)
Positive Sample:
Mouse thymus, Rat thymus
Cellular Localization:
Secreted.
Calculated MW:
13kDa
Observed MW:
13kDa
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5741
RRID
AB_2863761
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Parathyroid Hormone (PTH) Rabbit mAb (CAB9704) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Confocal imaging of paraffin-embedded Mouse thyroid tissue using Parathyroid Hormone (PTH) Rabbit mAb (CAB9704, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
