The Anti-Pan Tri-Methyl Lysine Antibody (CAB20145) is a valuable tool for researchers studying post-translational modifications of proteins, specifically focusing on lysine methylation. This antibody, produced through rabbit immunization, exhibits high specificity and sensitivity in detecting tri-methylated lysine residues in a wide range of protein samples.Lysine methylation is a common modification that plays a crucial role in regulating protein function, particularly in the context of epigenetics and gene expression. The Anti-Pan Tri-Methyl Lysine Antibody allows for the identification and analysis of tri-methylated lysines within proteins, providing insights into their roles in various cellular processes.
With its versatility and reliability in applications such as Western blotting and immunohistochemistry, this antibody is ideal for researchers investigating the impact of lysine methylation on protein function and the potential therapeutic implications in diseases such as cancer and metabolic disorders. By targeting tri-methylated lysine residues, this antibody enables researchers to deepen their understanding of protein regulation and signaling pathways, paving the way for advancements in biomedical research.
Product Name:
pan-Tri-Methyl lysine Rabbit Polyclonal Antibody
SKU:
CAB20145
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence containing Tri-methylated K.
Tested Applications:
WBIPChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 5μg-10μg of Chromatin
Positive Sample:
HeLa,MCF7,NIH/3T3,C6
Observed MW:
15-74kDa
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).The post-translational ε-amino lysine methylated proteins is an important reversible modification which plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription. Methylation of lysine residues is modulated by specific counteractive enzymes including lysine methylases (KMTs) and demethylases (KDMs). Lysine trimethylation occurs in both histones and non-histone substratres. It has become promising targets for discovery of anti-cancer drugs.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using pan-Tri-Methyl lysine Rabbit pAb (CAB20145) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
