The Anti-Pan Symmetric Di-Methyl Arginine Motif Antibody (CAB20794) is a versatile tool for researchers studying protein methylation and its implications in cellular functions. This polyclonal antibody, raised against the symmetric di-methyl arginine motif, is highly specific and sensitive in detecting proteins containing this modification.With applications in Western blot and immunofluorescence assays, this antibody facilitates the visualization and analysis of symmetric di-methyl arginine-modified proteins in various biological samples. Its reactivity with human samples provides researchers with a valuable tool for investigating the role of protein methylation in health and disease.The symmetrical di-methylation of arginine residues is a crucial post-translational modification that regulates diverse cellular processes, including gene expression, protein-protein interactions, and signal transduction.
By targeting this specific modification, the CAB20794 antibody enables researchers to explore the functional significance of protein methylation in a wide range of biological contexts.Whether studying epigenetic regulation, chromatin structure, or protein function, the Anti-Pan Symmetric Di-Methyl Arginine Motif Antibody offers a reliable and specific tool for unraveling the complex mechanisms underlying cellular physiology and pathology. Its versatility and sensitivity make it an essential reagent for researchers interested in the dynamic and multifaceted world of protein methylation.
A synthetic peptide corresponding to a sequence containing Symmetric Di-Methyl R.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
HeLa, HCT 116, MCF7, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Conjugate:
Unconjugated
Observed MW:
10-180kDa
Symmetric Dimethylarginine is a dimethylated derivative of L-arginine where the two methyl groups are attached to arginine in a symmetrical configuration. Symmetric dimethylarginine (SDMA) is formed when S-adenosylmethionine protein N-methyltransferases transfer one methyl group from S-adenosylmethionine to each of the two guanidine nitrogen groups of a single arginine residue in a protein. SDMA is released when the protein is degraded. SDMA doesn't bind nitric oxide synthase (NOS) but may noncompetitivelty inhibit nitric oxide (NO) synthesis by reducing L-arginine availability; it also may play a role in the modulation of cardiovascular homeostasis and renal function.
Purification Method:
Affinity purification
Clone Number:
ARC51197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Pan-SymmetricDi-MethylArginineMotif Rabbit mAb (CAB20794) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
