The Anti-Pan Succinyl K Antibody (CAB20503) is a valuable tool for researchers studying protein succinylation, a post-translational modification involved in various cellular processes. This antibody, generated in rabbits, specifically recognizes proteins containing succinyl lysine residues and is suitable for use in Western blot and immunoprecipitation experiments.Succinylation is a reversible modification that regulates protein function and stability, impacting diverse biological pathways such as metabolism, gene expression, and signal transduction. Dysregulation of succinylation has been implicated in diseases like cancer, metabolic disorders, and neurodegenerative conditions, highlighting the importance of studying this modification for therapeutic development.
The Anti-Pan Succinyl K Antibody enables researchers to investigate the role of succinylation in cellular physiology and disease pathogenesis, providing insights into potential therapeutic targets and treatment strategies. Its high specificity and sensitivity make it a valuable asset for proteomics and cellular biology studies aimed at uncovering the diverse functions of succinylated proteins.
Product Name:
Pan-Succinyl-K Rabbit Polyclonal Antibody
SKU:
CAB20503
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing Succinyl-K.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
293T,MCF7,C6,Mouse liver,Mouse heart
Conjugate:
Unconjugated
Observed MW:
10-150kDa
Succinylation of protein is a kind of post-translational modification of protein mainly occurring on lysine residues. Compared with methylation and acetylation, succinylation of lysine has a greater effect on protein structure and function. Sirt5 is an important de-succinylation modifying enzyme involved in the regulation of a variety of biological processes, such as reactive oxygen species defense, fatty acid metabolism and cell apoptosis
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan-Succinyl-K Rabbit pAb (CAB20503) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
