The Anti-Pan-Nitrific-γ Antibody (CAB20441) offered by AssayGenie is a valuable tool for research involving the Nitrific-γ protein, a key player in the nitrogen cycle and denitrification processes. This polyclonal antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for various experimental applications such as Western blotting.Nitrific-γ is essential for the conversion of nitrite to nitrate in bacteria, which is crucial for nitrogen cycling in aquatic and terrestrial ecosystems. This antibody specifically targets the Nitrific-γ protein, enabling researchers to detect and analyze its expression in different cell types and environmental samples.
Understanding the role of Nitrific-γ in nitrogen metabolism is vital for studying microbial ecology, environmental biogeochemistry, and nitrogen pollution control strategies.By utilizing the Anti-Pan-Nitrific-γ Antibody, researchers can further explore the functions of Nitrific-γ in diverse microbial communities and ecosystems, paving the way for innovative solutions in environmental science, agriculture, and biotechnology. Investigating the molecular mechanisms of Nitrific-γ can lead to new insights into nitrogen cycling processes and provide valuable information for sustainable resource management and ecosystem conservation efforts.
Product Name:
pan-Nitrific-Y Rabbit Polyclonal Antibody
SKU:
CAB20441
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence containing myristylated K.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
HeLa treated by Perxynitrate,C6 treated by Perxynitrate,NIH/3T3 treated by Perxynitrate
Conjugate:
Unconjugated
Observed MW:
10-250kDa
nan
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using pan-Nitrific-Y Rabbit pAb (CAB20441) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
