The Anti-Pan Mono-Methyl R R GG Motif Antibody (CAB18297) is a high-quality polyclonal antibody designed for research involving mono-methylation of the R R GG motif. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications such as immunoprecipitation and immunofluorescence.The R R GG motif is a common target for methylation, and understanding the role of mono-methylation in this motif is crucial for elucidating its function in cellular signaling pathways. The CAB18297 Antibody specifically recognizes mono-methylated R R GG motif, enabling precise detection and analysis of this modification in different cell types.
This antibody is validated for use in Western blot applications, providing researchers with a reliable tool for studying the role of mono-methylation in various biological processes.Overall, the CAB18297 Antibody is a valuable tool for researchers studying the role of mono-methylation in cellular signaling and regulation. Its high specificity and reactivity make it an essential reagent for research in the fields of epigenetics, cell biology, and cancer research.
Arginine methylation is a common posttranslational modification that is found on both histone and non-histone proteins. Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). The most prevalent is omega-NG,NG-dimethylarginine. Here, two methyl groups are placed on one of the terminal nitrogen atoms of the guanidino group; this derivative is commonly referred to as asymmetric dimethylarginine (ADMA). Two other derivatives occur at levels of about 20% to 50% that of ADMA. These include the symmetric dimethylated derivative, where one methyl group is placed on each of the terminal guanidino nitrogens and the monomethylated derivative with a single methyl group on the terminal nitrogen atom . These three derivatives are present on a multitude of distinct protein species in the cytoplasm, nucleus, and organelles of mammalian cells. Methylated arginine residues in proteins are often flanked by one or more glycine residues, but there are many exceptions to this general rule.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using pan-Mono-MethylR（R
