Pan Lactic acid-Lysine Rabbit Polyclonal Antibody (CAB18831)
The Anti-Pan Lactic Acid Lysine Antibody (CAB18831) is a valuable tool for researchers studying lactic acid lysine, a key component involved in cellular metabolism and energy production. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.Lactic acid lysine is commonly found in cells undergoing anaerobic metabolism and is known to play a role in processes like the Cori cycle and gluconeogenesis. By targeting this specific molecule, researchers can gain insights into metabolic pathways and potentially uncover new therapeutic targets for conditions related to cellular energy metabolism.
Furthermore, this antibody allows for the detection and analysis of lactic acid lysine in different cell types, making it suitable for a range of studies in areas such as biochemistry, physiology, and metabolism. With its ability to specifically bind to this molecule, the CAB18831 antibody is a valuable asset for advancing research in the fields of cellular biology and metabolic diseases.
Product Name:
Pan Lactic acid-Lysine Rabbit Polyclonal Antibody
SKU:
CAB18831
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing Pan Lactic acid-Lysine.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Positive Sample:
MCF7,NIH/3T3,C6
Histone lysine lactation (Kla) is a newly discovered histone modification that regulates gene expression in macrophages. In M1 macrophages, lactic acid is derived from incompletely oxidized glucose and then produces lactyl-CoA, which is transferred via acetyltransferase p300 to the lysine tail of the histone. This modification is abundant in gene promoter regions that lack acetylation and are associated with gene expression activation.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Pan Lactic acid-Lysine Rabbit pAb (CAB18831) at 1:400 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
