The Anti-Pan Crotonyl K Antibody (CAB20502) is a highly specific antibody designed for research involving crotonylation, a post-translational modification of proteins involved in gene regulation and other cellular processes. This antibody, produced in rabbits, demonstrates high reactivity with crotonylated lysine residues in various samples, making it a valuable tool for studying protein crotonylation in biological systems.Crotonylation is a newly discovered histone modification that plays a role in gene expression and chromatin remodeling. The Anti-Pan Crotonyl K Antibody binds specifically to crotonylated lysine residues, allowing for the detection and analysis of crotonylated proteins in various cell types and tissues. This antibody is validated for use in applications such as Western blot, immunoprecipitation, and immunofluorescence, providing researchers with a versatile tool for studying the role of protein crotonylation in various physiological and pathological processes.
Researchers in the fields of epigenetics, chromatin biology, and cancer biology will find the Anti-Pan Crotonyl K Antibody to be an invaluable resource for their studies. By targeting crotonylated proteins, this antibody enables the investigation of the impact of protein crotonylation on gene regulation, cellular signaling, and disease development. Enhance your research with the Anti-Pan Crotonyl K Antibody and uncover the role of protein crotonylation in biological processes.
Product Name:
Pan-Crotonyl-K Rabbit Polyclonal Antibody
SKU:
CAB20502
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing acetylated K.
Lysine crotonylation (Kcr) was first discovered about 10 years ago, as a PTM of histone. It was revealed that Kcr is an evolutionarily conserved and common PTM that occurs in both core histone and some non-histone proteins in a variety of organisms. Similar to other types of PTM, Kcr is a reversible modification. The classic histone acetyltransferases (HATs), p300/CBP, PCAF, and MOF, are responsible for most crotonylation events, while the histone deacetylases (HDACs) HDAC1/2/3 and SIRT1/2/3 reverse these reactions . The chromodomain protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone Kcr by reducing substrate supply. CDYL-regulated Kcr of RPA1 plays an important role in homologous recombination (HR)-mediated DNA repair (23), while HDAC-regulated histone crotonylation is reduced after DNA damage .
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan-Crotonyl-K Rabbit pAb (CAB20502) at 1:1000 dilution.HeLa and NIH/3T3 cells were treated by Sodium crotonate for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
