The p150CAF-1 Antibody (CAB8725) is a valuable tool for researchers studying the p150 component of the Chromatin Assembly Factor 1 (CAF-1) complex. This polyclonal antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.The p150CAF-1 complex plays a crucial role in chromatin assembly and maintenance, ensuring proper DNA replication and repair processes. By targeting the p150 subunit, this antibody allows for the detection and analysis of the CAF-1 complex in various cell types, providing valuable insights into chromatin dynamics and gene regulation.
Research into the p150CAF-1 complex has implications for understanding various biological processes, including cell proliferation, differentiation, and development. Dysregulation of chromatin assembly factors like CAF-1 has been implicated in diseases such as cancer, highlighting the importance of studying these complexes for potential therapeutic interventions.Overall, the p150CAF-1 Antibody (CAB8725) is a reliable tool for investigating the role of the CAF-1 complex in chromatin biology and its potential implications for human health and disease.
Product Name:
p150 CAF1 Rabbit Monoclonal Antibody
SKU:
CAB8725
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human p150 CAF1 (NP_005474.2).
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).
Purification Method:
Affinity purification
Gene ID:
10036
Clone Number:
ARC2011
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using p150 CAF1 Rabbit mAb (CAB8725) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
