The NUP62 Polyclonal Antibody (CAB19271) is a valuable tool for researchers studying nuclear pore complex proteins. NUP62 is a key component of the nuclear pore complex, which regulates the passage of molecules in and out of the nucleus. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the NUP62 protein, researchers can detect and analyze its expression in various cell types, providing insights into its role in nucleocytoplasmic transport and cellular function.
NUP62 is essential for the proper functioning of the nuclear pore complex, and its dysregulation has been implicated in various diseases, including cancer and neurological disorders. This antibody enables the study of NUP62 in the context of these diseases, offering potential insights into disease mechanisms and therapeutic targets. By understanding the functions of NUP62, researchers can uncover new pathways for drug development and treatments for diseases impacted by nuclear transport processes.
Product Name:
NUP62 Rabbit Monoclonal Antibody
SKU:
CAB19271
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 423-522 of human NUP62 (P37198).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
p62; IBSN; SNDI; NUP62
Positive Sample:
PC-3,NIH/3T3,Jurkat,Neuro-2a,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, cytoskeleton, nuclear pore complex, spindle pole.
Calculated MW:
53kDa
Observed MW:
70kDa
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.
Purification Method:
Affinity purification
Gene ID:
23636
Clone Number:
ARC2444
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using NUP62 Rabbit mAb (CAB19271) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
