The NUP153 Polyclonal Antibody (CAB2472) is a valuable tool for researchers studying nuclear pore complex proteins. NUP153 is an essential component of the nuclear pore complex, which regulates the transport of molecules in and out of the nucleus. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot and immunofluorescence applications.The NUP153 Polyclonal Antibody allows for the detection and analysis of NUP153 protein expression in various cell types, providing researchers with insights into the function and localization of this key nuclear pore complex protein.
Understanding the role of NUP153 in nucleocytoplasmic transport is crucial for unraveling fundamental processes in cell biology and gene regulation.With its high specificity and sensitivity, the NUP153 Polyclonal Antibody is a valuable tool for researchers investigating nuclear envelope dynamics, nucleocytoplasmic transport mechanisms, and the impact of nuclear pore complex proteins on cellular function. Its versatility and reliability make it an essential component of studies in molecular biology, cell biology, and nuclear transport pathways.
Product Name:
NUP153 Rabbit Monoclonal Antibody
SKU:
CAB2472
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUP153 (P49790).
Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
9972
Clone Number:
ARC1922
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using NUP153 Rabbit mAb (CAB2472) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
