NUF2 Rabbit Monoclonal Antibody (CAB17553)
- SKU:
- CAB17553
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- CDCA1
- CT106
- NUF2R
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Product Name: | NUF2 Rabbit Monoclonal Antibody |
| SKU: | CAB17553 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUF2 (Q9BZD4). |
| Sequence: | METL SFPR YNVA EIVI HIRN KILT GADG KNLT KNDL YPNP KPEV LHMI YMRA LQIV YGIR LEHF YMMP VNSE VMYP HLME GFLP FSNL VTHL DSFL PICR |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:100- 1:500 |
| Synonyms: | CDCA1; CT106; NUF2R; NUF2 |
| Positive Sample: | HeLa,293T,MCF7 |
| Conjugate: | Unconjugated |
| Cellular Localization: | cytosol, nucleoplasm |
| Calculated MW: | 54kDa |
| Observed MW: | 54kDa |
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
| Purification Method: | Affinity purification |
| Gene ID: | 83540 |
| Clone Number: | ARC2157 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using NUF2 Rabbit mAb (CAB17553) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s. |
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