The Anti-NUF2 Antibody (CAB17553) is a valuable tool for researchers studying the NUF2 protein, an essential component of the NDC80 kinetochore complex involved in chromosome segregation during cell division. This polyclonal antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blotting applications.NUF2 is crucial for proper chromosome alignment and segregation during mitosis, making it a key player in cell division and genome stability. Dysregulation of NUF2 has been linked to chromosome instability and various types of cancer, highlighting its potential as a target for therapeutic intervention.
By targeting the NUF2 protein, researchers can gain valuable insights into the mechanisms underlying cell division and chromosome segregation, as well as explore its potential role in cancer development and progression. The Anti-NUF2 Antibody provides a reliable tool for detecting and studying NUF2 in various cell types, making it an essential asset for studies in cell biology, cancer research, and drug discovery.
Product Name:
NUF2 Rabbit Monoclonal Antibody
SKU:
CAB17553
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUF2 (Q9BZD4).
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
Purification Method:
Affinity purification
Gene ID:
83540
Clone Number:
ARC2157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NUF2 Rabbit mAb (CAB17553) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
