The NPHS2 Polyclonal Antibody (CAB2302) is a valuable tool for researchers studying NPHS2, a protein involved in kidney function and development. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot experiments. By binding to the NPHS2 protein, researchers can detect and analyze its expression in various cell types, making it an ideal choice for studies in nephrology and kidney disease research.
NPHS2, also known as podocin, is a key component of the kidney filtration barrier, playing a crucial role in maintaining normal kidney function. Mutations in the NPHS2 gene have been linked to nephrotic syndrome, a rare kidney disorder characterized by proteinuria and edema. Understanding the function of NPHS2 is essential for unraveling the mechanisms underlying kidney diseases and developing targeted treatments for patients affected by these conditions.
Product Name:
NPHS2 Rabbit Monoclonal Antibody
SKU:
CAB2302
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 284-383 of human NPHS2 (Q9NP85).
cell-cell junction, cytoplasmic side of plasma membrane, endoplasmic reticulum, extracellular exosome, plasma membrane, slit diaphragm
Calculated MW:
42kDa
Observed MW:
35kDa
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7827
Clone Number:
ARC1907
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NPHS2 antibody (CAB2302) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
