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Nitro-Tyrosine Rabbit Polyclonal Antibody (CAB20506)

SKU:
CAB20506
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Nitro-Tyrosine Rabbit Polyclonal Antibody
SKU:CAB20506
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Chemical compounds corresponding to Nitro-Tyrosine.
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Positive Sample:HeLa treated by Perxynitrate,NIH/3T3 treated by Perxynitrate,C6 treated by Perxynitrate
Conjugate:Unconjugated
Observed MW:10-250kDa

Tyrosine nitration of proteins is one of the most important oxidative post-translational modifications in vivo. A major obstacle for its biochemical and physiological studies is the lack of efficient and chemoselective protein tyrosine nitration reagents.

Purification Method:Affinity purification
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Nitro-Tyrosine Rabbit pAb (CAB20506) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.NIH3T3 cells were treated by Perxynitrate（3mM）for 3 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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