The Anti-Nitro Tyrosine Antibody (CAB20506) is an essential tool for researchers studying protein modifications related to oxidative stress and inflammation. Nitro tyrosine is a marker of protein damage caused by reactive nitrogen species, and its detection is crucial for understanding the role of oxidative stress in various diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer.This polyclonal antibody, produced in rabbits, is highly specific to nitro tyrosine and has been validated for use in various applications, including immunohistochemistry and ELISA.
Its high reactivity with human samples makes it an excellent choice for studying disease mechanisms and evaluating the effectiveness of potential therapeutic interventions targeting oxidative stress.With the Anti-Nitro Tyrosine Antibody (CAB20506), researchers can confidently investigate the impact of oxidative stress on protein function and structure, paving the way for the development of innovative treatments that target oxidative damage in disease settings. Don't miss out on this valuable tool for your research needs in the fields of immunology, neurology, and oncology.
Product Name:
Nitro-Tyrosine Rabbit Polyclonal Antibody
SKU:
CAB20506
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Chemical compounds corresponding to Nitro-Tyrosine.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
HeLa treated by Perxynitrate,NIH/3T3 treated by Perxynitrate,C6 treated by Perxynitrate
Conjugate:
Unconjugated
Observed MW:
10-250kDa
Tyrosine nitration of proteins is one of the most important oxidative post-translational modifications in vivo. A major obstacle for its biochemical and physiological studies is the lack of efficient and chemoselective protein tyrosine nitration reagents.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Nitro-Tyrosine Rabbit pAb (CAB20506) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.NIH3T3 cells were treated by Perxynitrate（3mM）for 3 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
