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Neurofilament L Rabbit Monoclonal Antibody (CAB20269)

SKU:
CAB20269
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
NEFL
CMT1F
CMT2E
NF-L
NF68
NFL
PPP1R110
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Neurofilament L Rabbit Monoclonal Antibody
SKU:CAB20269
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2).
Sequence:SSGS LMPS LENL DLSQ VAAI SNDL KSIR TQEK AQLQ DLND RFAS FIER VHEL EQQN KVLE AELL VLRQ KHSE PSRF RALY EQEI RDLR LAAE DATN EKQA LQGE REGL EETL RNLQ ARYE EEVL SRED AEGR LMEA RKGA DEAA LARA ELEK RIDS LMDE ISFL KKVH EEEI AELQ AQIQ YAQI SVEM DVT
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:10000 - 1:100000 IHC-P,1:100 - 1:500 IF/ICC,1:50 - 1:200
Synonyms:NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110; Neurofilament L
Positive Sample:SH-SY5Y,
Conjugate:Unconjugated
Cellular Localization:axon, axon cytoplasm, cholinergic synapse, cytoplasm, cytosol, growth cone, neurofilament, neuromuscular junction, postsynaptic intermediate filament cytoskeleton, presynaptic intermediate filament cytoskeleton, Schaffer collateral - CA1 synapse
Calculated MW:62kDa
Observed MW:70kDa/

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Purification Method:Affinity purification
Gene ID:4747
Clone Number:ARC50056
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from SH-SY5Y cells using Neurofilament L Rabbit mAb(CAB20269) at 1:100000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time:30s.

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