The NELFCD Polyclonal Antibody (CAB4741) is a powerful tool for researchers studying NELFCD, a subunit of the NELF complex involved in the regulation of RNA polymerase II during transcription. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting.NELFCD plays a crucial role in the control of gene expression by pausing RNA polymerase II, ultimately influencing transcriptional regulation. Dysfunction of this complex has been linked to various diseases, making NELFCD an important target for further investigation in the fields of molecular biology and genetics.
By specifically binding to NELFCD, this antibody enables the detection and analysis of the protein in different cell types, providing valuable insights for researchers working on gene expression regulation and transcriptional control mechanisms. Its versatility and reliability make it an indispensable tool for studies related to gene transcription and its impact on cellular processes.
Product Name:
NELFCD Rabbit Polyclonal Antibody
SKU:
CAB4741
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-182 of human NELFCD (NP_945327.3).
The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.
Purification Method:
Affinity purification
Gene ID:
51497
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using NELFCD Rabbit pAb (CAB4741) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
