The NEK7 Polyclonal Antibody (CAB21088) is a valuable tool for researchers studying NEK7, a protein involved in cell cycle regulation and mitotic progression. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in various applications, including Western blotting.NEK7, a member of the NIMA-related kinase family, plays a crucial role in mitotic spindle assembly and chromosome segregation during cell division. Dysregulation of NEK7 has been linked to aberrant cell proliferation and tumorigenesis, highlighting its importance in cancer biology.
By targeting NEK7 with this antibody, researchers can gain insights into its function and potential therapeutic implications in oncology research.Overall, the NEK7 Polyclonal Antibody is a reliable tool for investigating the role of NEK7 in cellular processes and diseases, making it a valuable asset for studies in cell biology and cancer research.
Product Name:
NEK7 Polyclonal Antibody
SKU:
CAB21088
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1).
cytoplasm, microtubule organizing center, nucleoplasm, nucleus, spindle pole
Calculated MW:
35kDa
Observed MW:
32kDa
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.
Purification Method:
Affinity purification
Gene ID:
140609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NEK7 antibody (CAB21088) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
