The NEK7 Monoclonal Antibody (CAB19816) is a valuable tool for researchers interested in studying NEK7, a key player in the regulation of cell cycle progression and mitosis. This antibody, produced in mice, is highly specific for NEK7 in human samples and is optimized for use in various experimental applications, including Western blot and immunofluorescence.NEK7 is a member of the NIMA-related kinase family and plays a crucial role in centrosome duplication, microtubule organization, and mitotic progression. Dysregulation of NEK7 has been linked to various diseases, including cancer, making it a promising target for therapeutic intervention.
By using the NEK7 Monoclonal Antibody, researchers can study the expression and localization of NEK7 in different cell types and gain insights into its function in normal and disease states.Overall, the NEK7 Monoclonal Antibody (CAB19816) is a valuable tool for investigating the role of NEK7 in cellular processes and disease pathogenesis, with potential implications for the development of novel therapeutic strategies targeting NEK7 signaling pathways.
Product Name:
NEK7 Rabbit Monoclonal Antibody
SKU:
CAB19816
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 203-302 of human NEK7 (Q8TDX7).
cytoplasm, microtubule organizing center, nucleoplasm, nucleus, spindle pole, cytoskeleton, centrosome
Calculated MW:
35kDa
Observed MW:
32kDa
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.
Purification Method:
Affinity purification
Gene ID:
140609
Clone Number:
ARC2342
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NEK7 Rabbit mAb (CAB19816) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
