The NEK6 Polyclonal Antibody (CAB3536) is a valuable tool for researchers studying NEK6, a protein kinase involved in cell cycle regulation and mitosis. This antibody, generated in rabbits, exhibits high specificity for human samples and is suitable for use in Western blotting and immunohistochemistry applications. By binding to NEK6, this antibody enables the detection and analysis of the protein in various cell types, making it useful for studies in cell biology and cancer research.NEK6 is known to play a critical role in cell division and the maintenance of genomic stability.
Dysregulation of NEK6 has been implicated in various diseases, including cancer. Understanding the function of NEK6 is important for unraveling its role in tumorigenesis and identifying potential therapeutic targets. The NEK6 Polyclonal Antibody is a valuable tool for investigating the functions and pathways involving NEK6, providing insights into its potential as a target for cancer treatment strategies.
Product Name:
NEK6 Rabbit Monoclonal Antibody
SKU:
CAB3536
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human NEK6 (Q9HC98).
Sequence:
ETTA AHSL VGTP YYMS PERI HENG YNFK SDIW SLGC LLYE MAAL QSPF YGDK MNLF SLCQ KIEQ CDYP PLPG EHYS EKLR ELVS MCIC PDPH QRPD IGYV H
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:
SID6-1512; NEK6
Positive Sample:
293T,K-562,Mouse lung,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, Nucleus speckle, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
36kDa
Observed MW:
35kDa
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10783
Clone Number:
ARC2034
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using NEK6 Rabbit mAb (CAB3536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
