The Anti-NEDD4L Antibody (CAB9078) is a valuable tool for researchers studying NEDD4L, a key regulator of protein degradation and cellular signaling pathways. This antibody, produced in rabbits, is highly specific to human samples and has been rigorously validated for use in Western blot and immunohistochemistry applications. By binding to the NEDD4L protein, this antibody allows for the precise detection and analysis of NEDD4L expression in various cell types.NEDD4L, a member of the NEDD4 family of E3 ubiquitin ligases, is known for its role in ubiquitination and subsequent degradation of target proteins.
Its involvement in cellular processes such as endocytosis, cell growth, and immune response regulation makes it a promising target for research in areas like cancer biology, neurobiology, and immune system function. A better understanding of NEDD4L's function and regulation could lead to the development of novel therapeutic strategies for various diseases and conditions.
Product Name:
NEDD4-2 Rabbit Monoclonal Antibody
SKU:
CAB9078
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 876-975 of human NEDD4L (Q96PU5).
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
23327
Clone Number:
ARC1404
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NEDD4L antibody (CAB9078) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
