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NDUFAF1 Rabbit Monoclonal Antibody (CAB4007)

SKU:
CAB4007
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CGI-65
CGI65
CIA30
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:NDUFAF1 Rabbit Monoclonal Antibody
SKU:CAB4007
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFAF1 (Q9Y375).
Sequence:MALV HKLL RGTY FLRK FSKP TSAL YPFL GIRF AEYS SSLQ KPVA SPGK ASSQ RKTE GDLQ GDHQ KEVA LDIT SSEE KPDV SFDK AIRD EAIY HFRL LKDE IVDH WRGP EGHP LHEV LLEQ AKVV WQFR GKED LDKW TVTS DKTI GGRS EV
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:CGI65; CIA30; CGI-65; MC1DN11; NDUFAF1
Positive Sample:U2OS,293T,HepG2
Conjugate:Unconjugated
Cellular Localization:cytosol, mitochondrial inner membrane, mitochondrial matrix, mitochondrial respiratory chain complex I, mitochondrion
Calculated MW:38kDa
Observed MW:35kDa

This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.

Purification Method:Affinity purification
Gene ID:51103
Clone Number:ARC2091
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using NDUFAF1 Rabbit mAb (CAB4007) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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