The NDUFAF1 Polyclonal Antibody (CAB4007) is a valuable tool for researchers studying NDUFAF1, a key protein involved in mitochondrial function and energy production. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to the NDUFAF1 protein, this antibody enables accurate detection and analysis in various cell types, facilitating research in fields such as bioenergetics, metabolism, and mitochondrial diseases.NDUFAF1 is a critical component of complex I in the mitochondrial electron transport chain, playing a crucial role in ATP synthesis and cellular respiration.
Dysregulation of NDUFAF1 has been associated with various metabolic disorders and mitochondrial dysfunction, making it a promising target for therapeutic interventions. Understanding the functions and mechanisms of NDUFAF1 is essential for developing novel treatments for diseases related to mitochondrial impairment, including neurodegenerative disorders, metabolic syndromes, and aging-related conditions.
Product Name:
NDUFAF1 Rabbit Monoclonal Antibody
SKU:
CAB4007
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFAF1 (Q9Y375).
This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.
Purification Method:
Affinity purification
Gene ID:
51103
Clone Number:
ARC2091
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NDUFAF1 Rabbit mAb (CAB4007) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
