The Anti-NCF4 (p40-phox) Antibody (CAB0935) is a highly specific tool for researchers studying the NCF4 protein, also known as p40-phox, which plays a crucial role in the regulation of immune responses and oxidative burst in phagocytes. This antibody, raised in rabbits, is known for its high reactivity with human samples and is validated for use in Western blot applications.NCF4 is a key component of the phagocytic NADPH oxidase complex, essential for the production of reactive oxygen species in response to infections and inflammation. Dysregulation of NCF4 has been implicated in various inflammatory disorders, making it an important target for research in immunology and infectious diseases.
By binding specifically to the NCF4 protein, this antibody enables researchers to detect and analyze NCF4 expression in different cell types, providing valuable insights into its role in immune function and potential therapeutic targets for conditions characterized by abnormal immune responses. This antibody is an essential tool for scientists interested in exploring the intricate mechanisms of immune regulation and oxidative stress in health and disease.
Product Name:
NCF4 Rabbit Monoclonal Antibody
SKU:
CAB0935
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 240-339 of human NCF4/p40-phox (Q15080).
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
4689
Clone Number:
ARC2553
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using NCF4/p40-phox Rabbit mAb (CAB0935) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
