The Anti-Nanog Antibody (CAB3232) is a valuable tool for research involving Nanog, a key transcription factor involved in maintaining pluripotency and self-renewal in embryonic stem cells. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in various applications, including immunofluorescence and immunohistochemistry.Nanog plays a critical role in regulating the expression of genes involved in stem cell maintenance and differentiation, making it an important target for studies in regenerative medicine and developmental biology.
By binding specifically to the Nanog protein, this antibody enables precise detection and analysis in various cell types, allowing researchers to explore the mechanisms underlying stem cell pluripotency.Research into Nanog function is vital for advancing our understanding of stem cell biology and its potential applications in regenerative medicine and disease modeling. The Anti-Nanog Antibody provides researchers with a reliable tool for investigating the role of Nanog in cellular processes, offering new insights into the mechanisms governing stem cell fate and differentiation.
Product Name:
NANOG Rabbit Polyclonal Antibody
SKU:
CAB3232
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Nanog (NP_079141.2).
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
79923
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain, using Nanog Rabbit pAb (CAB3232) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
