N4-acetylcytidine/ ac4C Rabbit Polyclonal Antibody (CAB18806)
- SKU:
- CAB18806
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Frequently bought together:
Description
Product Name: | N4-acetylcytidine/ ac4C Rabbit Polyclonal Antibody |
SKU: | CAB18806 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Species independent |
Immunogen: | ac4C |
Tested Applications: | ELISA DB |
Recommended Dilution: | DB,1:500 - 1:2000 |
Here we describe a chemical method for nucleotide resolution sequencing of N4-acetylcytidine (ac4C), a highly conserved modified nucleobase whose formation is catalyzed by the essential cytidine acetyltransferase enzyme NAT10. This approach enables the sensitive, PCR-amplifiable detection of individual ac4C sites from nanograms of unfractionated cellular RNA. The sensitive and quantitative nature of this assay provides a powerful tool to understand how cytidine acetylation is targeted, profile RNA acetyltransferase dynamics, and validate the sites and stoichiometry of ac4C in novel RNA species
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |