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MRPS31 Monoclonal Antibody (CAB20932)

SKU:
CAB20932
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG
  • Western blot analysis of extracts of various cell lines, using MRPS31 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using MRPS31 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
$215 - $563
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MRPS31 Monoclonal Antibody
SKU:CAB20932
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 296-395 of human MRPS31 (Q92665).
Sequence:NGFE ELIQ WTKE GKLW EFPI NNEA GFDD DGSE FHEH IFLE KHLE SFPK QGPI RHFM ELVT CGLS KNPY LSVK QKVE HIEW FRNY FNEK KDIL KESN IQFN
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:S31mt; IMOGN38; MRP-S31; MRPS31
Positive Sample:Jurkat,HepG2,HeLa,Mouse heart,Rat liver
Conjugate:Unconjugated
Cellular Localization:Mitochondrion.
Calculated MW:45kDa
Observed MW:38kDa

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13.

Purification Method:Affinity purification
Gene ID:10240
Clone Number:ARC2914
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using MRPS31 antibody (CAB20932) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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