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MRPS25 Rabbit Polyclonal Antibody (CAB9083)

SKU:
CAB9083
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MRPS25
MRP-S25
RPMS25
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MRPS25 Rabbit Polyclonal Antibody
SKU:CAB9083
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 104-173 of human MRPS25 (NP_071942.1).
Sequence:KILG KNEE TLRE EEEE KKQL SHPA NFGP RKYC LREC ICEV EGQV PCPS LVPL PKEM RGKY KAAL KADA QD
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:RPMS25; COXPD50; MRP-S25; MRPS25
Positive Sample:HeLa,A-549,BxPC-3
Cellular Localization:mitochondrial inner membrane, mitochondrion
Calculated MW:20kDa
Observed MW:20kDa

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:64432
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using MRPS25 Rabbit pAb (CAB9083) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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