The MRPS25 Polyclonal Antibody (CAB9083) is a valuable tool for researchers studying MRPS25, a mitochondrial ribosomal protein essential for protein synthesis within the mitochondria. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the MRPS25 protein, this antibody enables accurate detection and analysis in various cell types, making it a valuable asset for investigations in molecular biology and mitochondrial function research.MRPS25 is a key player in mitochondrial protein synthesis and function, making it a crucial component in cellular metabolism and energy production.
Dysfunction of MRPS25 has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, highlighting its importance in various pathological conditions. Understanding the role of MRPS25 in these processes is essential for the development of targeted therapies and interventions aimed at correcting mitochondrial dysfunction and improving cellular health.Overall, the MRPS25 Polyclonal Antibody (CAB9083) offers researchers a reliable tool for studying the function and expression of MRPS25 in a variety of biological contexts, providing valuable insights into mitochondrial biology and its implications for human health and disease.
Product Name:
MRPS25 Rabbit Polyclonal Antibody
SKU:
CAB9083
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 104-173 of human MRPS25 (NP_071942.1).
Sequence:
KILG KNEE TLRE EEEE KKQL SHPA NFGP RKYC LREC ICEV EGQV PCPS LVPL PKEM RGKY KAAL KADA QD
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
RPMS25; COXPD50; MRP-S25; MRPS25
Positive Sample:
HeLa,A-549,BxPC-3
Cellular Localization:
mitochondrial inner membrane, mitochondrion
Calculated MW:
20kDa
Observed MW:
20kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
64432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS25 Rabbit pAb (CAB9083) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
