The Anti-MRP2/ABCC2 Antibody (CAB9528) is a valuable tool for research involving the MRP2/ABCC2 protein, a member of the ATP-binding cassette (ABC) transporter family that plays a crucial role in drug metabolism and detoxification. This polyclonal antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.MRP2/ABCC2 is primarily expressed in the liver, where it is involved in the transport of a wide range of endogenous and exogenous substances, including drugs, toxins, and metabolites. Dysregulation of MRP2/ABCC2 function has been implicated in drug resistance in cancer cells and various liver diseases.
Research into the mechanisms of MRP2/ABCC2 activity is essential for understanding drug metabolism and developing strategies to overcome drug resistance in cancer therapy.This antibody binds specifically to the MRP2/ABCC2 protein, allowing for the detection and analysis of its expression levels in various cell types and tissues. Its high specificity and sensitivity make it an ideal tool for studies in pharmacology, toxicology, and cancer research, providing valuable insights into the roles of MRP2/ABCC2 in health and disease.
Product Name:
MRP2 Rabbit Monoclonal Antibody
SKU:
CAB9528
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1446-1545 of human MRP2/ABCC2 (Q92887).
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
Purification Method:
Affinity purification
Gene ID:
1244
Clone Number:
ARC1619
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (CAB9528) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
