Anti-Mouse/Rat CD71 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse/Rat CD71 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously designed for in vivo applications. This antibody targets the CD71 protein, known for its vital role in cell proliferation and iron uptake, making it indispensable for research in cellular biology and related fields. With a rat IgG2a kappa isotype, this antibody ensures high purity and low endotoxin levels (<1.0 EU/mg), making it suitable for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for enhanced stability and efficacy.
Elevate your research with this dependable and versatile antibody.
CD71, also known as the transferrin receptor, is a glycoprotein abundantly expressed on the surface of proliferating cells and actively dividing cells. It plays a crucial role in mediating cellular iron uptake by binding and internalizing transferrin-bound iron, which is essential for DNA synthesis and cell growth. This characteristic makes CD71 an important marker for studying cell proliferation, cancer research, and iron metabolism.
Product Name:
Anti-Mouse/Rat CD71 In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0265
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
OX-26
Protein:
CD71
Product Type:
Monoclonal Antibody
Isotype:
IgG2a
Reactivity:
Mouse, Rat
Applications:
FC, IHC, In Vivo
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FC, IHC, In Vivo
Reactivity:
Mouse/Rat
Host Species:
Mouse
Specificity:
Clone OX-26 reacts with rat CD71 (TfR1).
Antigen Distribution:
CD71 is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors.
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD71 is a transferrin receptor on the cell surface that mediates the uptake of transferrin-iron complexes, thereby mediating cellular proliferation1. CD71 is a homodimeric glycoprotein containing 760 amino acids and it binds to diferric transferrin2. Binding by diferric transfferin causes CD71 to be internalized, and diferric iron is subsequently released3. CD71 is highly expressed on malignant cells, as iron uptake is necessary for aberrant cell proliferation4.
