Anti-Mouse RANKL (CD254) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse RANKL [CD254] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets the RANKL (Receptor Activator of Nuclear factor Kappa-Β Ligand) protein, pivotal in bone metabolism and immune response, making it indispensable for research in bone biology, immunology, and related fields. With a rat IgG2a isotype, it ensures exceptional purity and ultra-low endotoxin levels (<0.01 EU/μg), ideal for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in multiple sizes, it is formulated in endotoxin-free phosphate-buffered saline for stability and optimal performance.
Elevate your research with this dependable and versatile antibody.
RANKL (CD254) is a member of the tumor necrosis factor (TNF) superfamily, expressed on the surface of osteoblasts and activated T-cells. It is crucial in the regulation of bone remodeling through its interaction with the RANK receptor on osteoclasts, promoting their formation, function, and survival. Additionally, RANKL plays a significant role in the immune system by influencing T-cell and dendritic cell activity.
Product Name:
Anti-Mouse RANKL (CD254) In Vivo Antibody - Ultra Low Endotoxin
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, FC, In Vivo, IP, WB
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone IK22/5 recognizes an epitope on mouse RANKL.
Antigen Distribution:
RANKL is expressed on activated T cells and osteoclasts.
Immunogen:
Purified Recombinant Mouse RANKL (NSO-derived)
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
RANKL antibody, clone IK22/5, recognizes receptor activator of NF-κB ligand (RANKL), also known as CD254, TNF-related activation-induced cytokine (TRANCE), tumor necrosis factor ligand superfamily member 11 (TNFSF11), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF). RANKL is a type II transmembrane protein belonging to the TNF cytokine superfamily and binds to the signaling receptor, RANK, or decoy receptor, osteoprotegerin (OPG). RANKL is expressed on osteoclasts and is essential for osteoclast differentiation, activation, and survival1. RANKL is also expressed on activated T cells and regulates T cell-dendritic cell (DC) interactions, enhancing DC activation and survival2-3. In addition, RANKL is crucial for lymph node, bone marrow, and thymus development4. Treatment with denosumab, an antibody directed against RANKL, is approved for the treatment of patients at high risk for bone fractures, including osteoporosis, and has shown anti-tumor effects5.
Protein:
RANKL
Ligand/Receptor:
RANK, OPG
Research Area:
Cell Biology, Immunology
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
