Anti-Mouse I-A/I-E (MHC Class II) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse I-A/I-E (MHC Class II) In Vivo Antibody - Ultra-Low Endotoxin from Assay Genie**, a highly specific monoclonal antibody crafted for in vivo research applications. This antibody targets the I-A/I-E molecules, essential components of the MHC class II complex, making it invaluable for studies in immunology, particularly those focusing on antigen presentation and immune response modulation. With a hamster IgG isotype, it ensures high purity and ultra-low endotoxin levels (<0.01 EU/μg), optimized for sensitive experimental setups like ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various quantities, it is formulated in phosphate-buffered saline to maintain stability and efficacy.
Elevate your research with the precision and reliability of this versatile antibody. **The I-A/I-E molecules are part of the MHC class II family, predominantly expressed on the surface of antigen-presenting cells such as B cells, dendritic cells, and macrophages. These molecules are pivotal in the immune system, facilitating the presentation of extracellular peptides to CD4+ T-helper cells, thus orchestrating adaptive immune responses.** --- Feel free to let me know if you'd like any modifications to the description!
Product Name:
Anti-Mouse I-A/I-E (MHC Class II) In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0356
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
M5/114.15.2
Protein:
I-A/I-E â‹… MHC class II
Product Type:
Monoclonal Antibody
Isotype:
IgG2b
Reactivity:
Mouse
Applications:
B, FC, IHC FF, In Vivo, IP, PhenoCycler®
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, FC, IHC FF, In Vivo, IP, PhenoCycler®
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.
Antigen Distribution:
I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes.
Immunogen:
C57BL/6 Spleen Cells
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6.
Protein:
I-A/I-E â‹… MHC class II
Ligand/Receptor:
CD3/TCR, CD4
Research Area:
Immunology, Innate Immunity
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
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