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Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin

SKU:
IVMB0178
Product Type:
In Vivo Monoclonal Antibody
Clone:
A4C8.1-Do9
Protein:
MHC Class I
Isotype:
Mouse IgG1 kappa
Reactivity:
Mouse
Synonyms:
Class I major histocompatibility antigen H-2Kd
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Low Endotoxin
Host Species:
Mouse
Applications:
FC
WB
$150 - $3,864
Frequently bought together:

Description

system_update_altTechnical Datasheet

Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin

Introducing the Anti-Mouse H-2Kd MHC Class I In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications.

This antibody targets the H-2Kd molecule, an important component in the major histocompatibility complex (MHC) class I that is essential for presenting endogenous antigens to CD8+ T-cells, thereby playing a pivotal role in immune surveillance and response. Featuring a rat IgG2a kappa isotype, this antibody ensures high purity and low endotoxin levels (<1.0 EU/mg), making it ideal for applications such as ELISA, flow cytometry, immunohistochemistry, and other relevant assays. Available in various sizes, it is formulated in phosphate-buffered saline to ensure optimal stability and efficacy. Enhance your immunological research with this reliable and versatile antibody. H-2Kd is a glycoprotein expressed on the surface of nucleated cells in mice, contributing to the presentation of antigenic peptides to cytotoxic T lymphocytes (CTLs). This interaction is crucial for the immune system to recognize and eliminate cells expressing foreign antigens, such as those infected by pathogens or transformed into cancer cells.

Product Name:Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin
Product Code:IVMB0178
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:A4C8.1-Do9
Protein:MHC Class I
Product Type:Monoclonal Antibody
Synonyms:Class I major histocompatibility antigen H-2Kd
Isotype:Mouse IgG1 κ
Reactivity:Mouse
Applications:FC, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:FC, WB
Recommended Usage:FC
The suggested concentration for clone A4C8.1-Do9 antibody for staining cells in flow cytometry is ≤ .25 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Mouse
Specificity:Clone A4C8.1-Do9 recognizes an epitope on mouse MHC class I H-2K haplotype d.
Antigen Distribution:H-2Kd is ubiquitously expressed on nucleated cells from mice of the H-2Kd haplotype, including BALB/c.
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

H-2Kd antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype d (H-2Kd). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kd plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Technical Datasheet:View
Protein:MHC Class I
Research Area:Immunology, Innate Immunity
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