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Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin

SKU:
IVMB0010
Product Type:
In Vivo Monoclonal Antibody
Clone:
Y-3
Protein:
MHC Class I
Isotype:
Mouse IgG2b kappa
Reactivity:
Mouse
Synonyms:
H-2K
H-2 class I histocompatibility antigen
K-B alpha chain
H-2K(B)
H-2K(K)
H-2K(Q)
H-2K(R)
H-2K(S)
Beta-2-microglobulin
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Mouse
Applications:
FA
ICC
IP
WB
€268 - €3,920
Frequently bought together:

Description

system_update_altTechnical Datasheet

Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin

Introducing the Anti-Mouse H-2Kb MHC Class I [Y-3] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a high-specificity monoclonal antibody tailored for in vivo research applications.

This antibody targets the H-2Kb MHC Class I protein, a critical element in immune response regulation, making it indispensable for studies in immunology and related disciplines. Featuring a rat IgG2a isotype, it ensures exceptional purity and ultra-low endotoxin levels (<0.01 EU/µg), suitable for ELISA, flow cytometry, immunoprecipitation, and other assays. Available in multiple sizes, this antibody is formulated in phosphate-buffered saline for maximal stability and performance. Elevate your research with this dependable and versatile antibody. H-2Kb MHC Class I molecules are essential components of the immune system, predominantly expressed on the surface of nucleated cells. They play a pivotal role in presenting endogenous antigens to CD8+ T cells, facilitating the immune system's ability to recognize and respond to intracellular pathogens and abnormal cells. This antibody enables researchers to effectively study antigen presentation, immune response modulation, and various other aspects of immunological function in murine models.

Product Name:Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0010
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:Y-3
Protein:MHC Class I
Product Type:Monoclonal Antibody
Synonyms:H-2K, H-2 class I histocompatibility antigen, K-B alpha chain, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin
Isotype:Mouse IgG2b κ
Reactivity:Mouse
Immunogen:Con A stimulated spleen cells from BALB.B mice
Applications:FA, ICC, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0009Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low EndotoxinLow
IVMB0010Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:FA, ICC, IP, WB
Reactivity:Mouse
Host Species:Mouse
Specificity:Clone Y-3 recognizes an epitope on mouse MHC class I H-2K haplotypes b, k, q, r, s, but not d.
Antigen Distribution:H-2K is ubiquitously expressed on nucleated cells.
Immunogen:Con A stimulated spleen cells from BALB.B mice
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

H-2K antibody, clone Y-3, recognizes the major histocompatibility complex (MHC) class I H-2K haplotypes b, k, q, r, s, but not d. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2K plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Technical Datasheet:View
Protein:MHC Class I
Ligand/Receptor:CD3/TCR, CD4
Research Area:Immunology, Innate Immunity
Product Thumbnail
Mouse IgG2b Isotype Control
Clone MPC-11
Isotype Mouse IgG2b
Endotoxin Level Ultra Low Endotoxin
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