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Anti-Mouse CD90 (Thy-1) [T24/31] In Vivo Antibody - Ultra Low Endotoxin

SKU:
IVMB0158
Product Type:
In Vivo Monoclonal Antibody
Clone:
T24/31
Protein:
CD90
Isotype:
Rat IgG
Reactivity:
Mouse
Synonyms:
T25
CD90
Thy-1
Thy1.1
Thy1.2
Thy-1.2
Research Area:
T Cell Depletion
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Rat
Applications:
Depletion
FA
FC
WB
$322 - $4,704
Frequently bought together:

Description

system_update_altTechnical Datasheet

Anti-Mouse CD90 (Thy-1) [T24/31] In Vivo Antibody - Ultra Low Endotoxin

Introducing the Anti-Mouse CD90 (Thy-1) [T24/31] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a meticulously crafted monoclonal antibody tailored for in vivo applications. This antibody specifically targets the CD90 (Thy-1) protein, a critical surface glycoprotein involved in cellular signaling and immune responses, making it a valuable tool for research in immunology, neuroscience, and stem cell biology. Exhibiting a mouse IgG2a isotype, it guarantees extraordinary purity and ultra low endotoxin levels (<1.0 EU/mg), thus ensuring minimal interference in your assays. Suitable for a range of applications including ELISA, flow cytometry, immunohistochemistry, and other analytical techniques, it is provided in a phosphate-buffered saline solution to maintain stability and effectiveness.

Elevate the quality and reliability of your research with this exceptional antibody. CD90 (Thy-1) is an essential cell surface glycoprotein expressed on various cell types such as T-cells, thymocytes, neurons, and hematopoietic stem cells. It plays an integral role in cell-cell and cell-matrix interactions, influencing processes like adhesion, migration, and differentiation. Its versatility and importance across multiple biological processes make the Anti-Mouse CD90 (Thy-1) [T24/31] an indispensable resource for scientists seeking to advance their studies in related fields.

Product Name:Anti-Mouse CD90 (Thy-1) [T24/31] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0158
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:T24/31
Protein:CD90
Product Type:Monoclonal Antibody
Synonyms:T25, CD90, Thy-1, Thy1.1, Thy1.2, Thy-1.2
Isotype:Rat IgG
Reactivity:Mouse
Applications:Depletion, FA, FC, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0158Anti-Mouse CD90 (Thy-1) [T24/31] In Vivo Antibody - Ultra Low EndotoxinUltra Low
IVMB0161Anti-Mouse CD90 (Thy-1) [T24/31] In Vivo Antibody - Low EndotoxinLow
Applications:Depletion, FA, FC, WB
Recommended Usage:FC
The suggested concentration for this T24/31 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications.
Reactivity:Mouse
Host Species:Rat
Specificity:CloneT24/31 recognizes a non-polymorphic determinant on mouse CD90 (Thy1.1 and Thy1.2 alloantigens).
Antigen Distribution:CD90 is expressed by thymocytes, peripheral T cells, myoblasts, epidermal cells, and keratinocytes.
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD90 is a 28-30 kD GPI-linked membrane glycoprotein and is part of the Ig superfamily. It interacts with CD45 in signal transduction. CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that CD90 binds with β2 and β3 integrins and is involved in the inhibition of hematopoietic stem cells proliferation and differentiation, as well as the regulation of cell adhesion and neurite outgrowth. It can be used as a marker for various stem cells, such as hematopoietic stem cells, and for the axonal processes of mature neurons. For use in FACS, CD90 is a popular surface marker for stem cells in combination with other markers such as CD34. There are two alleles for CD90 in mice that differ by one amino acid. The difference being that CD90.1 (Thy1.1) has an arginine and CD90.2 (Thy1.2) has a glutamine at position 108. CD90.2 is more prevalent and is expressed in most mice strains. CD90.1 is only expressed by a select few mice strains including AKR/J and PL strains. CD90.2 is a 25-35 kD GPI-anchored membrane glycoprotein. Like CD90, it is also in the Ig superfamily, interacts with CD45, and has involvement in signal transduction. The function of CD90.2 is thought to play roles in cognition, axon growth, T lymphocyte function, and apoptosis. CD90 acts as tumor suppressor for some tumors due to its action in upregulating thrombospondin, SPARC (osteonectin), and fibronectin. On the other hand, it has been suspected to aid in the spread of circulating melanoma cells. Regarding prostate cancer, CD90 has therapeutic potential for specific drug targeting due to its expression in cancer associated stroma, but not in normal stroma.

Technical Datasheet:View
Protein:CD90
Ligand/Receptor:CD45
Research Area:Immunology, Stem Cell

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