Anti-Mouse CD4 [YTS191] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD4 [YTS191] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo research. This antibody specifically targets the CD4 protein, a pivotal component in the immune system, making it indispensable for immunological research and related applications. With a rat IgG2b isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), making it ideal for use in ELISA, flow cytometry, immunohistochemistry, and numerous other assays.
Available in various sizes, it is formulated in phosphate-buffered saline to ensure stability and effectiveness. Elevate your research with the reliability and versatility of this antibody.
CD4 is a glycoprotein expressed on the surface of immune cells such as T-helper cells, monocytes, macrophages, and dendritic cells. It is essential for the activation of T-cells through its interaction with major histocompatibility complex (MHC) class II molecules, playing a crucial role in the immune response.
Product Name:
Anti-Mouse CD4 (Clone YTS191) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0330
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
YTS191
Protein:
CD4
Product Type:
Monoclonal Antibody
Isotype:
IgG2b k
Reactivity:
Mouse
Applications:
Depletion, FC, IHC FF, In Vivo, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
Depletion, FC, IHC FF, In Vivo, WB
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone YTS191 recognizes an epitope on mouse CD4.
Antigen Distribution:
CD4 is expressed on most thymocytes, MHC class II-restricted T cells, a subset of NKT cells, and subsets of dendritic cells and macrophages.
Immunogen:
Unknown
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD4 antibody, clone YTS191, recognizes CD4, a 58 kDa type I transmembrane glycoprotein of the Ig superfamily. CD4 is expressed by the majority of thymocytes, MHC class II-restricted T cells (helper T cells and immunosuppressive regulatory T cells), and subsets of natural killer T (NKT) cells, dendritic cells (DCs) and macrophages1-3. On T cells, CD4 is a co-receptor of the T cell receptor (TCR) and interacts with major histocompatibility complex (MHC) II molecules on antigen-presenting cells (APCs). CD4 contributes to T cell development and selection and enhances TCR-dependent signaling by up to 100-fold through the accumulation of Lck4-6. CD4 also contributes to the activation of NKT, macrophages, and DCs7,8.
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