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Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin

SKU:
IVMB0027
Product Type:
In Vivo Monoclonal Antibody
Clone:
2.4G2
Protein:
CD32/CD16
Isotype:
Rat IgG2b
Reactivity:
Mouse
Synonyms:
Fc-gamma R III/II
Ly-17
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
Blocking
CODEX
FA
FC
IHC FF
IP
WB
€125 - €3,220
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin
Product Code:IVMB0027
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:2.4G2
Protein:CD32/CD16
Product Type:Monoclonal Antibody
Synonyms:Fcγ R III/II, Ly-17
Isotype:Rat IgG2b
Reactivity:Mouse
Immunogen:Sorted pre-B cells
Applications:B, FA, FC, IHC FF, IP, PhenoCycler®, WB
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity: ≥95% monomer by analytical SEC
>95% by SDS Page
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0027Anti-Mouse CD32/CD16 In Vivo Antibody - Low EndotoxinLow
IVMB0028Anti-Mouse CD32/CD16 In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, CODEX®, FA, FC, IHC FF, IP, WB
Recommended Usage: FC
The suggested concentration for this 2.4G2 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
WB
The suggested concentration for this 2.4G2 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:Mouse
Host Species:Rat
Specificity:Clone 2.4G2 recognizes the FcγIII and FcγII receptors.
Antigen Distribution: These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.
Immunogen:Sorted pre-B cells
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity: ≥95% monomer by analytical SEC
>95% by SDS Page
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

Technical Datasheet:View
Protein:CD32/CD16
Function:Low affinity receptors for IgG
Ligand/Receptor:IgG
Research Area:Immunology, Innate Immunity
Product Thumbnail
Rat IgG2b Isotype Control
Clone 1-2
Isotype Rat IgG2b
Endotoxin Level Low Endotoxin
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