Anti-Mouse CD252 (OX40L) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD252 (OX40L) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets mouse CD252, also known as OX40 ligand (OX40L), which plays a critical role in T-cell proliferation and survival, making it indispensable for immunological research. With a rat IgG2a isotype, this antibody ensures exceptional purity and low endotoxin levels (<1.0 EU/mg), making it suitable for a variety of applications including ELISA, flow cytometry, and in vivo studies.
Available in multiple sizes, it is formulated in phosphate-buffered saline (PBS) to maintain stability and efficacy. Enhance your research endeavors with this reliable and versatile antibody.
CD252 (OX40L) is a membrane-bound glycoprotein expressed on the surface of antigen-presenting cells such as B cells, dendritic cells, and macrophages. It plays a pivotal role in the immune system by interacting with OX40 (CD134) on T-cells, thereby influencing T-cell activation, survival, and memory formation.
Product Name:
Anti-Mouse CD252 (OX40L) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0316
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
RM134L
Protein:
CD252
Product Type:
Monoclonal Antibody
Synonyms:
OX 40 Ligand, OX40L, CD134L, TNFSF4
Isotype:
IgG2b k
Reactivity:
Mouse
Applications:
B, FC, IHC FF, In Vivo, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, FC, IHC FF, In Vivo, WB
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone RM134L recognizes an epitope on mouse CD252.
Antigen Distribution:
CD252 is expressed on activated B cells and antigen presenting cells.
Immunogen:
Rat NRK-52E cell line transfected with mouse OX-40L gene
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.
